Difference between revisions of "Part:BBa C0061"
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<partinfo>BBa_C0061 short</partinfo> | <partinfo>BBa_C0061 short</partinfo> | ||
| − | Synthesizes [[ | + | Synthesizes [[3OC6HSL|3OC<sub>6</sub>HSL]], which binds to LuxR. |
The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the [[Lux]] repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound [[AHL|HSL]]. This complex binds to a palindromic site on the promoter, increasing the rate of transcription. | The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the [[Lux]] repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound [[AHL|HSL]]. This complex binds to a palindromic site on the promoter, increasing the rate of transcription. | ||
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Enzyme for creating acyl-homoserine lactones from normal cell metabolites. | Enzyme for creating acyl-homoserine lactones from normal cell metabolites. | ||
| − | All LuxI proteins direct the synthesis of specific acyl-HSLs and show sequence similarity. | + | All LuxI proteins direct the synthesis of specific [[AHL|acyl-HSLs]] and show sequence similarity. |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_C0061 SequenceAndFeatures</partinfo> | <partinfo>BBa_C0061 SequenceAndFeatures</partinfo> | ||
Revision as of 22:01, 20 July 2006
autoinducer synthetase for AHL
Synthesizes 3OC6HSL, which binds to LuxR.
The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux repressor, LuxR. Two molecules of LuxR protein form a complex with two molecules the signalling compound HSL. This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
Usage and Biology
Enzyme for creating acyl-homoserine lactones from normal cell metabolites. All LuxI proteins direct the synthesis of specific acyl-HSLs and show sequence similarity.
Sequence and Features
Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 637
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]