Difference between revisions of "Part:BBa K1073001"

Revision as of 10:51, 28 September 2013

Inducible promoter of the rhl quorum sensing system with RBS

This construct is derived from BBa_R0071 and BBa_B0032. It combines the inducible promoter of the rhl Quorum sensing system of P. aeruginosa which is activated by RhlR in combination with N-buturyl homoserine lactone (N-buturyl-HSL) with an RBS.

Usage and Biology

The device can be cloned in front of a desired gene of interest in order to induce its expression by N-buturyl-HSL. For further information seeThe inducible Promotor from Pseudomonas aeruginosa is activated by RhlR complexed with the specific autoinducer N-buturyl-HSL produced by RhlI. The induction of the promoter in P. aeruginosa leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003].

BBa_R0071.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
-The inducible Promotor from ''Pseudomonas aeruginosa'' is activated by RhlR complexed with the specific autoinducer N-buturyl-HSL produced by RhlI. The induction of the promoter
-in ''P. aeruginosa'' leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003].
 The device can be cloned in front of a desired gene of interest in order to induce its expression by N-buturyl-HSL.
-For further information see <partinfo>BBa_R0071</partinfo>.
+For further information seeThe inducible Promotor from ''Pseudomonas aeruginosa'' is activated by RhlR complexed with the specific autoinducer N-buturyl-HSL produced by RhlI. The induction of the promoter
+in ''P. aeruginosa'' leads to the activation of transcription of different virulence-associated traits in the cell [Medina et al., 2003].
+ <partinfo>BBa_R0071</partinfo>.