Difference between revisions of "Part:BBa K1119008"
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<partinfo>BBa_K1119008 short</partinfo> | <partinfo>BBa_K1119008 short</partinfo> | ||
− | In characterization of Mitochondrial Leader Sequence (BBa_K1119001), negative control was made by GFP generator that does not contains the CDS of MLS (BBa_K1119008). The translation unit was driven by CMV promoter (BBa_K1119006) and terminated by hGH polyA signal (BBa_K404108). | + | In characterization of Mitochondrial Leader Sequence ([[Part:BBa_K1119001|BBa_K1119001]]), negative control was made by GFP generator that does not contains the CDS of MLS ([[Part:BBa_K1119008|BBa_K1119008]]). The translation unit was driven by CMV promoter ([[Part:BBa_K1119006|BBa_K1119006]]) and terminated by hGH polyA signal ([[Part:BBa_K404108|BBa_K404108]]). |
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Revision as of 11:49, 26 September 2013
CMV promoter - GFP - hGH polyA tail
In characterization of Mitochondrial Leader Sequence (BBa_K1119001), negative control was made by GFP generator that does not contains the CDS of MLS (BBa_K1119008). The translation unit was driven by CMV promoter (BBa_K1119006) and terminated by hGH polyA signal (BBa_K404108).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 614
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 628
Illegal AgeI site found at 1369 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1772
Illegal BsaI.rc site found at 1274