Difference between revisions of "Part:BBa K1151000:Design"

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===Source===
 
===Source===
  
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It was amplified by PCR from the genomic sequence of Helicobacter pylori G27.  
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We received this piece by Alberto Danielli, a researcher in molecular biology at the Department of Pharmacology and Biotechnology at the University of Bologna. He sent us the gene encoding NikR (from the genome of H. pylori G27) inserted into a plasmid pET-15b.
  
 
===References===
 
===References===

Revision as of 09:51, 26 September 2013


Nickel-responsive pleiotropic regulator (HpNikR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

We received this piece by Alberto Danielli, a researcher in molecular biology at the Department of Pharmacology and Biotechnology at the University of Bologna. He sent us the gene encoding NikR (from the genome of H. pylori G27) inserted into a plasmid pET-15b.

References

1. Muller et al, Hierarchical regulation of the NikR-mediated nickel response in Helicobacter pylori. Nucleic Acids Res. 2011; 39(17):7564-75.

2. Dian et al, Structural Basis of the Nickel Response in Helicobacter pylori: Crystal Structures of HpNikR in Apo and Nickel -bound States. J Mol Biol. 2006; 25;361(4):715-30.

3. Dosanjh et Michel, Microbial nickel metalloregulation: NikRs for nickel ions. Curr Opin Chem Biol. 2006 (2):123-30.

4. Ernst et al, The nickel-responsive regulator NikR controls activation and repression of gene transcription in Helicobacter pylori . Infect Immun. 2005; 73(11):7252-8.