Difference between revisions of "Part:BBa K1088012"

Revision as of 00:30, 4 October 2013

E. coli dxs (lac promoter without lac inhibitor)

This part has the dxs gene derived from E. coli (BBa_K118000) controlled by the lac promoter and has a strong RBS. The part was build to increase the flow through the MEP pathway, and to test whether Dxs derived from E. coli or B. subtilis increased the flow through the MEP pathway the most in E. coli.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 946

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 <partinfo>BBa_K1088012 short</partinfo>
-This part has the ''dxs'' gene derived from ''E. coli'' (BBa_K118000) controlled by the lac promoter and has a strong RBS. The part was build to increase the flow through the MEP pathway.
+This part has the ''dxs'' gene derived from ''E. coli'' (BBa_K118000) controlled by the lac promoter and has a strong RBS. The part was build to increase the flow through the MEP pathway, and to test whether Dxs derived from ''E. coli'' or ''B. subtilis'' increased the flow through the MEP pathway the most in ''E. coli''.
-Fluorescence activated cell sorting (FACS) was used to examine the expression profile of a similar part (BBa_K1088008) with linker-GFP attached to the end of Dxs derived from ''B. subtilis''. The part was examined in the K-12 MG1655 (natural levels of LacI) and KG22 (overexpression of LacI from chromosome) strains. The experiment proved that overexpression of LacI is required for repression of the lac promoter (and therefore also control of the regulation).
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