Difference between revisions of "Part:BBa K1045012:Design"

(→‎Design Notes)
(→‎Source)
Line 10: Line 10:
 
===Source===
 
===Source===
  
−
To be continued
+
The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence originated from the Parts Registry page of [[Part:BBa_J23110|BBa_J23110]] and the sequence of the DarR operator found upstream of DarR (Zhang ''et al''., 2013).
  
 
===References===
 
===References===

Revision as of 17:35, 4 October 2013

Promoter with DarR operator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was constructed using a hybridization oligo composed of BBa_J23110, followed by an XbaI/SpeI scar and the DarR operator sequence.

Source

The hybridization oligos mentioned under "Design Notes" were purchased from Sigma-Aldrich. The sequence originated from the Parts Registry page of BBa_J23110 and the sequence of the DarR operator found upstream of DarR (Zhang et al., 2013).

References