Difference between revisions of "Part:BBa K1014014"
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<partinfo>BBa_K1014014 short</partinfo>
 
<partinfo>BBa_K1014014 short</partinfo>
  
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At the beginning, Ptet promoter is on and HrpS forming rate is max,.So HrpS is adequate at that time. Once treated with protein TetR,concentration of HrpS is dropped to a low level. While there will not be HrpR unless treated with IPTG. Only when both HrpS and HrpR are at a high level, can the promoter hrpL trigger the transcription of following genes. This device function like a digital 'AND' gate.<br />
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At the beginning, Ptet promoter is on and HrpS forming rate is max,.So HrpS is adequate at that time. Once treated with protein TetR,concentration of HrpS is dropped to a low level. While there will not be HrpR unless treated with IPTG. Only when both HrpS and HrpR are at a high level, can the promoter hrpL trigger the transcription of following genes. This device function like a digital 'AND' gate.
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<table cellpadding="2" border="1px" cellspacing="0" align="center" width="70%">
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<br />
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<caption><p align="justify"><b>Table 3</b> Tecan Safire II Parameters</p></caption>
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<table cellpadding="3" border="1px" cellspacing="0" align="center" width="70%">
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<tr><td><b>Excitation (nm)</b></td><td>585</td></tr>
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<caption><p align="justify"><b>Table 1</b> Relationship between input(TetR and IPTG) and output(RFP) </p></caption>
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<tr><td><b>Emission (nm)</b></td><td>610</td></tr>
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<tr><td><b>TetR</b></td><td><b>IPTG</b></td><td><b>RFP</b></td></tr>
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<tr><td><b>Excitation bandwidth (nm)</b></td><td>10</td></tr>
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<tr><td><b>TetR</b></td><td><b>IPTG</b></td><td><b>RFP</b></td></tr>
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<tr><td><b>Emission bandwidth (nm)</b></td><td>10</td></tr>
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<tr><td><b>TetR</b></td><td><b>IPTG</b></td><td><b>RFP</b></td></tr>
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<tr><td><b>Gain</b></td><td>63</td></tr>
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<tr><td><b>TetR</b></td><td><b>IPTG</b></td><td><b>RFP</b></td></tr>
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<tr><td><b>Number of reads</b></td><td>10</td></tr>
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<tr><td><b>TetR</b></td><td><b>IPTG</b></td><td><b>RFP</b></td></tr>
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<tr><td><b>Integration Time (microseconds)</b></td><td>40</td></tr>
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</table>
 
</table>
 
<br />
 
<br />

Revision as of 08:15, 26 September 2013

hrpL AND Gate device

At the beginning, Ptet promoter is on and HrpS forming rate is max,.So HrpS is adequate at that time. Once treated with protein TetR,concentration of HrpS is dropped to a low level. While there will not be HrpR unless treated with IPTG. Only when both HrpS and HrpR are at a high level, can the promoter hrpL trigger the transcription of following genes. This device function like a digital 'AND' gate.

Table 1 Relationship between input(TetR and IPTG) and output(RFP)

TetRIPTGRFP
TetRIPTGRFP
TetRIPTGRFP
TetRIPTGRFP
TetRIPTGRFP



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 618
    Illegal AgeI site found at 205
    Illegal AgeI site found at 211
    Illegal AgeI site found at 259
    Illegal AgeI site found at 825
    Illegal AgeI site found at 850
    Illegal AgeI site found at 1417
    Illegal AgeI site found at 1423
  • 1000
    COMPATIBLE WITH RFC[1000]