Difference between revisions of "Part:BBa K1139020"
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We confirmed that M13 genome with two modifications related to our design kept plaque forming activity. One is replacement of the promoter for g2p protein with a constitutive promoter, PlacIq (<partinfo>BBa_I14032</partinfo>). The other is accommodation of pSB3K3 backbone. Even though the plasmid has two different types of replication origins, M13 origin and pSB3 origin, this plasmid (<partinfo>BBa_K1139020</partinfo>) formed plaque. In contrast, construction intermediates without a promoter for g2p coding sequence (Promoterless-M13 + Plac, Promoterless-M13 + PlacGFP <partinfo>BBa_K1139022</partinfo>) could not form plaque.<br> | We confirmed that M13 genome with two modifications related to our design kept plaque forming activity. One is replacement of the promoter for g2p protein with a constitutive promoter, PlacIq (<partinfo>BBa_I14032</partinfo>). The other is accommodation of pSB3K3 backbone. Even though the plasmid has two different types of replication origins, M13 origin and pSB3 origin, this plasmid (<partinfo>BBa_K1139020</partinfo>) formed plaque. In contrast, construction intermediates without a promoter for g2p coding sequence (Promoterless-M13 + Plac, Promoterless-M13 + PlacGFP <partinfo>BBa_K1139022</partinfo>) could not form plaque.<br> | ||
| − | [[Image:Titech2013_parts_K1139020_Fig1.jpg|thumb|left|500px|'''Fig. 1.''' PlacIQ-M13-Plac-GFP on pSB3 (BBa_K1139020)]] | + | [[Image:Titech2013_parts_K1139020_Fig1.jpg|thumb|left|500px|'''Fig. 1.''' PlacIQ-M13-Plac-GFP on pSB3 (<partinfo>BBa_K1139020</partinfo>)]] |
[[Image:Titech2013_parts_K1139020_Fig2.jpg|thumb|left|500px|'''Fig. 2.''' Promoterless-M13-Plac-GFP on pSB3 (<partinfo>BBa_K1139022</partinfo>)]] | [[Image:Titech2013_parts_K1139020_Fig2.jpg|thumb|left|500px|'''Fig. 2.''' Promoterless-M13-Plac-GFP on pSB3 (<partinfo>BBa_K1139022</partinfo>)]] | ||
Revision as of 11:30, 26 September 2013
PlacIq-M13-Plac-GFP on pSB3
We confirmed that M13 genome with two modifications related to our design kept plaque forming activity. One is replacement of the promoter for g2p protein with a constitutive promoter, PlacIq (BBa_I14032). The other is accommodation of pSB3K3 backbone. Even though the plasmid has two different types of replication origins, M13 origin and pSB3 origin, this plasmid (BBa_K1139020) formed plaque. In contrast, construction intermediates without a promoter for g2p coding sequence (Promoterless-M13 + Plac, Promoterless-M13 + PlacGFP BBa_K1139022) could not form plaque.
Fig. 1. PlacIQ-M13-Plac-GFP on pSB3 (BBa_K1139020)
Fig. 2. Promoterless-M13-Plac-GFP on pSB3 (BBa_K1139022)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 145
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 6071
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 7335