Difference between revisions of "Part:BBa K1166000"

Revision as of 22:20, 27 September 2013

FNR-HIP1

HIP-1 drives gene expression under both acute and chronic hypoxia, but not under normoxia (Mengesha, et al., 2006). This part is composed of the HIP-1 promoter with the FNR expression cassette upstream for a stronger hypoxic regulation, this promoter will start translation in coding sequences 3’ downstream in a hypoxic environment.

Reference

Mengesha A, Dubois L, Lambin P, Landuyt W, Chiu RK, Wouters BG, Theys J. (2006). Development of a flexible and potent hypoxia-inducible promoter for tumor-targeted gene expression in attenuated Salmonella. Cancer Biol Ther. 5(9):1120-8.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1166000 short</partinfo>
-Composed of the HIP-1 promoter with the FNR expression cassette upstream for a stronger hypoxic regulation, this promoter will start translation in coding sequences 3’ downstream in a hypoxic environment.
+HIP-1 drives gene expression under both acute and chronic hypoxia, but not under normoxia (Mengesha, et al., 2006). This part is composed of the HIP-1 promoter with the FNR expression cassette upstream for a stronger hypoxic regulation, this promoter will start translation in coding sequences 3’ downstream in a hypoxic environment.
-'''Note:''' It’s important that your part doesn’t contain a stop codon nor a terminator since your protein will be fused at the C-terminus.
+==Reference==
+Mengesha A, Dubois L, Lambin P, Landuyt W, Chiu RK, Wouters BG, Theys J. (2006). Development of a flexible and potent hypoxia-inducible promoter for tumor-targeted gene expression in attenuated Salmonella. Cancer Biol Ther. 5(9):1120-8.
 <!-- Add more about the biology of this part here