Difference between revisions of "Part:BBa K1140006:Design"

Revision as of 03:23, 26 September 2013

pTet + 37 oC RNA thermometer + mCherry (LVA)

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 63
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 676
Illegal AgeI site found at 788
1000
COMPATIBLE WITH RFC[1000]

Design Notes

The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.

Source

Obtained synthetically. The RNA thermosensor sequence was obtained and adapted from [2] (u6 synthetic RNA thermometer). The mCherry coding sequence was optimized for E. coli codon usage. The pTet promoter is identical to the sequence found in part BBa_R0040.

References

Elowitz, MB and Leibler, S, (2000). A synthetic oscillatory network of transcriptional regulators, Nature, 20;403(6767):335-8.

Neupert J, Karcher D, Bock R (2008) Design of simple synthetic RNA thermometers for temperature- controlled gene expression in Escherichia coli. "Nucleic Acids Res, 36(19):e124.

@@ Line 11: / Line 11: @@
 Obtained synthetically.
-The RNA thermosensor sequence was obtained and adapted from [1] (u6 synthetic RNA thermometer).
+The RNA thermosensor sequence was obtained and adapted from [2] (u6 synthetic RNA thermometer).
 The mCherry coding sequence was optimized for ''E. coli'' codon usage.
 The pTet promoter is identical to the sequence found in part BBa_R0040.