Difference between revisions of "Part:BBa F2620"

Revision as of 18:24, 15 September 2006

3OC₆HSL -> PoPS Receiver

A transcription factor [LuxR] that is active in the presence of cell-cell signaling molecule 3OC6HSL is controlled by an operator [TetR]. Device input is 3OC6HSL. Device output is PoPS produced at a LuxR-regulated operator.

Usage and Biology

Full PoPS output at high 3OC6HSL levels and high plasmid copy [e.g., Part:pSB1A2] results in a reduced cell growth rate (see Load section). If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.

Column 1	Column 2

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1004

Device Characteristics

control	R0040
device_type	Receiver
family	-NA-
latency	Seconds
proteins	LuxR, TetR
signalling_molecule	3OC₆HSL
switch_point	2nM

Get the device [http://openwetware.org/wiki/Image:F2620.de2-ania.ppt#filelinks datasheet]

Transfer Function

Latency

Specificity

[http://openwetware.org/images/9/93/Transfer.jpg Specificity preliminary data]

Stability

[http://openwetware.org/images/b/b9/StabilitySlideCropped.jpg Stability preliminary data]

@@ Line 6: / Line 6: @@
 ===Usage and Biology===
 Full [[PoPS]] output at high [[3OC6HSL]] levels and high plasmid copy [e.g., [[Part:pSB1A2]]] results in a reduced cell growth rate (see Load section).  If used in a cell containing TetR then a second input signal such as [http://openwetware.org/wiki/ATc aTc] can be used to produce a logical AND function.
+{|
+!Column 1
+!Column 2
+|-
+|[[Image:BC-TransferCurve-3.png|300px]]
+|[[Image:BC-Specificity-1.png|300px]]
+|-
+|[[Image:BC-Latency-2.png|300px]]
+|align=center|[[Image:BC-Stability-1.png|225px]]
+|}
 <span class='h3bb'>Sequence and Features</span>