Difference between revisions of "Part:BBa J23117:Experience"
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| − | [[File:J23117.jpg|420px|thumb|left|Fig. 1. RFP and qRT-PCR promoter analyses.]] | + | [[File:J23117.jpg|420px|thumb|left|'''Fig. 1. RFP and qRT-PCR promoter analyses'''.]] |
| − | [[File:promoterGoe.jpg|420px|thumb|right|Fig. 2. Microscopic promoter analyses on single cell level]] | + | [[File:promoterGoe.jpg|420px|thumb|right|'''Fig. 2. Microscopic promoter analyses on single cell level''' <br /> |
| + | P1: [[Part: BBa_J23117|BBa_J23117]], P2: [[Part:BBa_J23116|BBa_J23116]], P3: [[Part:BBa_J23110|BBa_J23110]], P4: [[Part:BBa_J23118|BBa_J23118]], P5: control ]] | ||
Revision as of 14:39, 25 September 2013
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Applications of BBa_J23117
Evaluation of Anderson promoter J23117 in B. subtilis by iGEM-Team LMU-Munich 2012
This Anderson promoter was evaluated without fused RFP with the lux operon as a reporter in B. subtilis. See the new BioBrick BBa_K823013 without RFP and have a look at the [http://2012.igem.org/Team:LMU-Munich/Data/Anderson Data] from the evaluation in B. subtilis.
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UNIQ42282bc4155acc01-partinfo-00000000-QINU UNIQ42282bc4155acc01-partinfo-00000001-QINU
Evaluation of Anderson promoter J23117 in E. coli by [http://2013.igem.org/Team:Goettingen iGEM Göttingen 2013]
Shown here:
Upper two pictures: Growth curves of promoter strains on the left, growth curves of control strains on the right. Three biological replicates are shown.
Middle two pictures: RFP/OD600 of promoter strains on the left, RFP/OD600 of control strains on the right. Three biological replicates are shown.
Bottom three pictures: qRT PCR promoter analyses in three different growth phases. Promoters are normalised against BBa_J23117 .
Promotor 1:BBa_J23117
Promoter 2:BBa_J23116
Promoter 3:BBa_J23110
Promoter 4:BBa_J23118
The promoter strength was measured by using the reporter gene rfp.
Three different approaches were used: 1. RFP measurement, 2. qRT_PCR analyses and 3. single cell microscopy.
Our results from these approaches showed that BBa_J23177 has the lowest promoter activity compared to BBa_J23116, BBa_J23110 and BBa_J23118.
P1: BBa_J23117, P2: BBa_J23116, P3: BBa_J23110, P4: BBa_J23118, P5: control