Difference between revisions of "Part:BBa J23117:Experience"
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[[File:J23117.jpg|420px|thumb|left|Fig. 1. Microscopic promoter analyses.]] | [[File:J23117.jpg|420px|thumb|left|Fig. 1. Microscopic promoter analyses.]] | ||
− | [[File:promoterGoe.jpg|420px|thumb|right|Fig. 2. RFP and qRT-PCR promoter analyses | + | [[File:promoterGoe.jpg|420px|thumb|right|Fig. 2. RFP and qRT-PCR promoter analyses]] |
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Revision as of 14:19, 25 September 2013
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Applications of BBa_J23117
Evaluation of Anderson promoter J23117 in B. subtilis by iGEM-Team LMU-Munich 2012
This Anderson promoter was evaluated without fused RFP with the lux operon as a reporter in B. subtilis. See the new BioBrick BBa_K823013 without RFP and have a look at the [http://2012.igem.org/Team:LMU-Munich/Data/Anderson Data] from the evaluation in B. subtilis.
User Reviews
UNIQ664c22e2148ddeda-partinfo-00000000-QINU UNIQ664c22e2148ddeda-partinfo-00000001-QINU
Evaluation of Anderson promoter J23117 in E. coli by [http://2013.igem.org/Team:Goettingen iGEM Göttingen 2013]
Shown here:
Upper two pictures: Growth curves of promoter strains on the left, growth curves of control strains on the right. Three biological replicates are shown.
Middle two pictures: RFP/OD600 of promoter strains on the left, RFP/OD600 of control strains on the right. Three biological replicates are shown.
Bottom three pictures: qRT PCR promoter analyses in three different growth phases. Promoters are normalised against BBa_J23117 .
Promotor 1:BBa_J23117
Promoter 2:BBa_J23116
Promoter 3:BBa_J23110
Promoter 4:BBa_J23118