Difference between revisions of "Part:BBa K1157021:Experience"

Revision as of 11:39, 27 September 2013

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Please enter how you used this part and how it worked out.

Applications of BBa_K1157021

This construct is a positive feedback circuit that expresses both C-4 AHL receptor RhlR and green fluorescence protein when AHL-binded RhlR combines to its promoter sequence. The design indicates that the promoter sequence is placed in front of the circuit, while the constitutive promoter is placed in the middle of the circuit. NYMU_Taipei assisted the functional testing by using ELISA plate reader to read GFP fluorescence intensity against C-4 AHL throughout time. The biosensor is cultured overnight and diluted until OD=0.1. We detect the fluorescence intensity from 0 to 4 hours. The results are as follows.File:Rhl positive feedback circuit

User Reviews

UNIQ52990137c1f76f13-partinfo-00000000-QINU UNIQ52990137c1f76f13-partinfo-00000001-QINU

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 ===Applications of BBa_K1157021===
-This construct is a positive feedback circuit that expresses both C-4 AHL receptor RhlR and green fluorescence protein when AHL-binded RhlR combines to its promoter sequence. The design indicates that the promoter sequence is placed in front of the circuit, while the constitutive promoter is placed in the middle of the circuit. We used ELISA plate reader to read GFP fluorescence intensity against C-4 AHL throughout time. The biosensor is cultured overnight and diluted until OD=0.1. We detect the fluorescence intensity from 0 to 4 hours. The results are as follows.[[File:Rhl positive feedback circuit]]
+This construct is a positive feedback circuit that expresses both C-4 AHL receptor RhlR and green fluorescence protein when AHL-binded RhlR combines to its promoter sequence. The design indicates that the promoter sequence is placed in front of the circuit, while the constitutive promoter is placed in the middle of the circuit. NYMU_Taipei assisted the functional testing by using ELISA plate reader to read GFP fluorescence intensity against C-4 AHL throughout time. The biosensor is cultured overnight and diluted until OD=0.1. We detect the fluorescence intensity from 0 to 4 hours. The results are as follows.[[File:Rhl positive feedback circuit]]
 ===User Reviews===