Difference between revisions of "Part:BBa K1045002:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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This part was amplified from ''Bacillus subtilis'' genomic DNA and ligated into the ''EcoRI'' and ''PstI'' digested pSB1C3 backbone (see part BBa_K1045004). It was then further ''SpeI'' and ''EcoRI'' digested and ligated with the ''Xba'' and ''EcoRI'' digested CFP (BBa-E0020). altogether this composes part BBa_K1045002.
+
This part was amplified from ''Bacillus subtilis'' genomic DNA and ligated into the ''EcoRI'' and ''PstI'' digested pSB1C3 backbone (see part BBa_K1045004). It was then further ''SpeI'' and ''EcoRI'' digested and ligated with the ''Xba'' and ''EcoRI'' digested CFP (BBa-E0020). Altogether this composes part BBa_K1045002.
  
 
===Source===
 
===Source===

Revision as of 12:44, 25 September 2013

CFP Reporter under control of the c-di-AMP-dependent YdaO Riboswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone (see part BBa_K1045004). It was then further SpeI and EcoRI digested and ligated with the Xba and EcoRI digested CFP (BBa-E0020). Altogether this composes part BBa_K1045002.

Source

References

Peter Y Watson & Martha J Fedor (2012) “The ydaO motif is an c-di-AMP-sensing riboswitch in Bacillus subtilis“, Nature Chemical Biology No. 8, pp. 963-965