Difference between revisions of "Part:BBa K1017602"
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− | <br>From chart 1, we can clearly found that the time to reach same O.D. 0.8 various as the temperature changes. The expression of mGFP under 37°C and 42°C reached a higher OD value in less time than the expression | + | <br>From chart 1, we can clearly found that the time to reach same O.D. 0.8 various as the temperature changes. The expression of mGFP under 37°C and 42°C reached a higher OD value in less time than the expression under 32°C and 27°C. This demonstrates the fact that 37°C RBS functions more efficiently above or at 37°C. On the other hand, the fact that 37°C RBS leads to moderate expression even under 37°C degree might be a problem, since then we cannot make sure that only single gene is expressed under 37°C. |
[[File:NCTU 37RBS result.png|center|600px|Chart 1. 37°C RBS efficiency under different temperatures.]] | [[File:NCTU 37RBS result.png|center|600px|Chart 1. 37°C RBS efficiency under different temperatures.]] | ||
Revision as of 12:34, 25 September 2013
37℃ RBS + mGFP
This biobrick is comprised of 37 degrees Celsius RBS and mGFP. 37℃ RBS, a kind of RBS which is controlled by temperature. mGFP is a green fluorescent protein. In our project, we use this part to conduct the test of temperature regulated-system. Pcons promoter is a constitutive promoter. This promoter can be used to tune the expression level of constitutively expressed parts. By adding a Pcons promoter and controlling temperature , we can measure the O.D. value to determine the level of GFP's expression.
Mechanism
Data
From chart 1, we can clearly found that the time to reach same O.D. 0.8 various as the temperature changes. The expression of mGFP under 37°C and 42°C reached a higher OD value in less time than the expression under 32°C and 27°C. This demonstrates the fact that 37°C RBS functions more efficiently above or at 37°C. On the other hand, the fact that 37°C RBS leads to moderate expression even under 37°C degree might be a problem, since then we cannot make sure that only single gene is expressed under 37°C.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]