Difference between revisions of "Part:BBa K1045002"
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This part is a reporter system. It was constructed from a riboswitch from Bacillus subtilis with a native promoter and native rbs (part BBa_K1045004) and a CFP reporter. It works as a sensor of c-di-AMP, which enhances the terminating efficiency in the translation of the RNA by ribosomes. Therefore in the presence of c-di-AMP the system should be hindered and not be glowing. | This part is a reporter system. It was constructed from a riboswitch from Bacillus subtilis with a native promoter and native rbs (part BBa_K1045004) and a CFP reporter. It works as a sensor of c-di-AMP, which enhances the terminating efficiency in the translation of the RNA by ribosomes. Therefore in the presence of c-di-AMP the system should be hindered and not be glowing. | ||
− | In combination with the diadenylate cyclase it might show further | + | In combination with the diadenylate cyclase (like a further BBa_K1045003) it might show further interaction. |
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Revision as of 12:03, 25 September 2013
CFP Reporter under control of the c-di-AMP-dependent YdaO Riboswitch
This part is a reporter system. It was constructed from a riboswitch from Bacillus subtilis with a native promoter and native rbs (part BBa_K1045004) and a CFP reporter. It works as a sensor of c-di-AMP, which enhances the terminating efficiency in the translation of the RNA by ribosomes. Therefore in the presence of c-di-AMP the system should be hindered and not be glowing. In combination with the diadenylate cyclase (like a further BBa_K1045003) it might show further interaction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]