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Revision as of 12:02, 25 September 2013

Ph-B0031-sfGFP-Terminator (HcaR)



Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 113


Construction and data

For the biosensor circuit Ph/J23114-HcaR, we created a library of RBS (Ribosome Binding Site) including B0031, B0032 and B0034 for tuning the expression level of reporter gene sfGFP. K1031420 is composed of three elements, Pc promoter activated by HcaR, RBS B0031[1] and reporter gene sfGFP (Fig 1a). Induction ratio was calculated from fluorescence intensity, showing the performance of K1031420(Fig 1b)

Fig 1 Construction and tunning of reporter circuit Pc-B0031-sfGFP (a) The orange arrow represents Pc promoter for HbpR. The green oval stands for RBS B0031[2]. sfGFP coding sequence is shown with dark blue, while terminator B0015[3] is in dark red. (b) Horizontal axis represents biosensor circuit adopting different combination of Pc promoter and RBS. Vertical axis stands for induction ratio. The dashed box refers to data for reporter circuit Pc-B0031-sfGFP in collocation with biosensor circuit J23114-HcaR.