Difference between revisions of "Part:BBa J37016:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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na
 
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*The purpouse for building and testing this part is so we can characterize the production of AHL-lactonase
 +
*The Aiia gene has been replaced with GFP to allow direct measurement of protein production
 +
*From this data we can create a transfer function where AHL is the input and GFP the output
 +
*Making the assumpion that AHL-lactonase is transcribed at the same rate as GFP we can use the transfer curve to extract parameters to allow characterization of the sensing part of the predator
 +
*The part will be used to form the sensing part of the final predator construct
  
 
===Source===
 
===Source===

Revision as of 17:38, 29 October 2006


Predator Molecule Generator Sensing Test (GFP output)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1532


Design Notes

  • The purpouse for building and testing this part is so we can characterize the production of AHL-lactonase
  • The Aiia gene has been replaced with GFP to allow direct measurement of protein production
  • From this data we can create a transfer function where AHL is the input and GFP the output
  • Making the assumpion that AHL-lactonase is transcribed at the same rate as GFP we can use the transfer curve to extract parameters to allow characterization of the sensing part of the predator
  • The part will be used to form the sensing part of the final predator construct

Source

na

References