Difference between revisions of "Part:BBa K1061002"
 
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===Usage and Biology===
 
===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1061002 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1061002 SequenceAndFeatures</partinfo>
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====functional experiment====
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The death-induce effect of RIP 1
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[[Image:Suicidegene-rip1.png|thumb|center|800px|Expression of RIP 1 in two kinds of cell lines, with GFP control.]]
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This is the pre-experiment we do. We also confirm the death-induction effect quantitively by FCM.Detail can be seen
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in our wiki http://2013.igem.org/Team:SYSU-China/Project/Results
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The data will be uploaded here at the following days.
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====kill range====
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For each suicide gene, it is important to know which cancer cells and normal cells it will kill.So that people can speficially choose gene that will be functional in their project when duelling about cancer. And we define this new feature for each suicide gene that we submitted. The list will be changed due to more and more experiment will be done. We highly recommend people to adopt this characteristic as important characteristic of suicide gene.
  
 
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Latest revision as of 17:19, 26 September 2013

RIP 1

Receptor interacting protein 1, a serine/threonine kinase that play an important role in cell apoptosis and necrosis. Overexpression of RIP 1 in cancer cells may induce cell death[1].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1437
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1242
    Illegal BsaI.rc site found at 1519
    Illegal SapI.rc site found at 511
    Illegal SapI.rc site found at 1696

functional experiment

The death-induce effect of RIP 1

Expression of RIP 1 in two kinds of cell lines, with GFP control.

This is the pre-experiment we do. We also confirm the death-induction effect quantitively by FCM.Detail can be seen in our wiki http://2013.igem.org/Team:SYSU-China/Project/Results The data will be uploaded here at the following days.

kill range

For each suicide gene, it is important to know which cancer cells and normal cells it will kill.So that people can speficially choose gene that will be functional in their project when duelling about cancer. And we define this new feature for each suicide gene that we submitted. The list will be changed due to more and more experiment will be done. We highly recommend people to adopt this characteristic as important characteristic of suicide gene.

References:

[1] [1]Olivier Micheau, Jürg Tschopp et al,2003, Induction of TNF Receptor I-Mediated Apoptosis via Two Sequential Signaling Complexes,Cell,114:181-190