Difference between revisions of "Part:BBa K1045000"
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The important information: The operator is not in the pSBC13 Vector but instead in the pBluescript -KS plasmid. This plasmid provides an Ampicillin resistance. Furthermore it has got a pUC origin.
 
The important information: The operator is not in the pSBC13 Vector but instead in the pBluescript -KS plasmid. This plasmid provides an Ampicillin resistance. Furthermore it has got a pUC origin.
  
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(Reference: Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096)
 
  
 
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Revision as of 07:04, 25 September 2013

DarR operator

This part is the binding sequence of the c-di-AMP-dependent regulatory protein DarR. When bound by DarR, it is supposed to prevent the downstream information to be transcribed. The important information: The operator is not in the pSBC13 Vector but instead in the pBluescript -KS plasmid. This plasmid provides an Ampicillin resistance. Furthermore it has got a pUC origin.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]