Difference between revisions of "Part:BBa K1045000"
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The important information: The operator is not in the pSBC13 Vector but instead in the pBluescript -KS plasmid. This plasmid provides an Ampicillin resistance. Furthermore it has got a pUC origin. | The important information: The operator is not in the pSBC13 Vector but instead in the pBluescript -KS plasmid. This plasmid provides an Ampicillin resistance. Furthermore it has got a pUC origin. | ||
| − | (Reference: Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a | + | (Reference: Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096) |
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Revision as of 22:41, 24 September 2013
DarR operator
This part is the binding sequence of the c-di-AMP-dependent regulatory protein DarR. When bound by DarR, it is supposed to prevent the downstream information to be transcribed. The important information: The operator is not in the pSBC13 Vector but instead in the pBluescript -KS plasmid. This plasmid provides an Ampicillin resistance. Furthermore it has got a pUC origin.
(Reference: Lei Zhang, Weihui Li, and Zheng-Guo He (2013) “DarR, a TetR-like Transcriptional Factor, Is a Cyclic Di-AMP-responsive Repressor in Mycobacterium smegmatis”, THE JOURNAL OF BIOLOGICAL CHEMISTRY VOL. 288, NO. 5, pp. 3085–3096)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]