Difference between revisions of "Part:BBa K1114701:Design"
 
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This is a BioBrick adaptation of the Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses RFP. It contains the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114005">J23104_AB</a>, <a href="https://parts.igem.org/Part:BBa_K1114108:Design">BCD8_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114211">E0040m_CD</a>, and <a href="https://parts.igem.org/Part:BBA_K783000">B0015_DE</a> within the <a href="https://parts.igem.org/Part:pSB1C3">pSB1C3</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
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This is a BioBrick adaptation of the Level 1 <a href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> device that constitutively expresses RFP. It contains the Level 0 parts <a href="https://parts.igem.org/Part:BBa_K1114005">J23104_AB</a>, <a href="https://parts.igem.org/Part:BBa_K1114108:Design">BCD8_BC</a>, <a href="https://parts.igem.org/Part:BBa_K1114211">E1010m_CD</a>, and <a href="https://parts.igem.org/Part:BBA_K783000">B0015_DE</a> within the <a href="https://parts.igem.org/Part:pSB1C3">pSB1C3</a> backbone. In the given sequence the internal fusion sites are incorrectly duplicated.
 
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.  
 
The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.  
  

Latest revision as of 22:07, 24 September 2013

BioBrick of Level 1 MoClo RFP Reporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 11
    Illegal NheI site found at 34
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 694
    Illegal AgeI site found at 806
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a BioBrick adaptation of the Level 1 MoClo device that constitutively expresses RFP. It contains the Level 0 parts J23104_AB, BCD8_BC, E1010m_CD, and B0015_DE within the pSB1C3 backbone. In the given sequence the internal fusion sites are incorrectly duplicated. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.


Source

Construct of MoClo Level 0 parts derived from iGEM Distribution Kit.

References