Difference between revisions of "Part:BBa K1114106"

Revision as of 19:46, 24 September 2013

Bicistronic Design RBS 1, MoClo Format with BC fusion sites

This part is a MoClo adapted bicistronic design (BCD) element, which is a 5' UTR regulatory part from Mutalik et al., 2013. It contains two Shine-Dalgarno sequences instead of the standard one in ribosomal binding units such as BBa_B0034. From the library of BCDs, this is a MoClo version of BCD1 (BioFAB # apFAB681) and we obtained the sequence data for this part from the BioFAB.

This BCD1 is flanked by two MoClo fusion sites, B on the 5' end and C on the 3' side.

The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. See Level 0 pages for further information.

This is Figure 1A from Mutalik et al., 2013 showing the basic structure of their BCD design. The bicistronic design (BCD) with its two Shine-Dalgarno motifs (SD1 and SD2)is shown in gray.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <html>
-This part is a <a href="href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> adapted bicistronic design (BCD) element, which is a 5' UTR regulatory part from <a href="http://www.nature.com/nmeth/journal/v10/n4/abs/nmeth.2404.html">Mutalik et al., 2013</a>. From the library of BCDs, this is a MoClo version of BCD1 (BioFAB # apFAB681) and we obtained the sequence data for this part from the <a href="http://biofab.org/data">BioFAB</a>.
+This part is a <a href="href="http://2013.igem.org/Team:BostonU/MoCloChara">MoClo</a> adapted bicistronic design (BCD) element, which is a 5' UTR regulatory part from <a href="http://www.nature.com/nmeth/journal/v10/n4/abs/nmeth.2404.html">Mutalik et al., 2013</a>. It contains two Shine-Dalgarno sequences instead of the standard one in ribosomal binding units such as <a href="https://parts.igem.org/Part:BBa_B0034">BBa_B0034</a>. From the library of BCDs, this is a MoClo version of BCD1 (BioFAB # apFAB681) and we obtained the sequence data for this part from the <a href="http://biofab.org/data">BioFAB</a>.
 <br><br>
@@ Line 13: / Line 13: @@
 <center><img src="https://static.igem.org/mediawiki/parts/1/1d/BCD_Fig1.png" width="600px"></a></center>
 <br><br>
-This is Figure 1A from <a href="http://www.nature.com/nmeth/journal/v10/n4/abs/nmeth.2404.html">Mutalik et al., 2013</a> showing the basic structure of their BCD design.
+This is Figure 1A from <a href="http://www.nature.com/nmeth/journal/v10/n4/abs/nmeth.2404.html">Mutalik et al., 2013</a> showing the basic structure of their BCD design. The bicistronic design (BCD) with
+its two Shine-Dalgarno motifs (SD1 and SD2)is shown in gray.
 </ul></ul>