Difference between revisions of "Part:BBa K1150002"
NightworkMax (Talk | contribs) |
NightworkMax (Talk | contribs) |
||
Line 27: | Line 27: | ||
|} | |} | ||
− | Krüppel-associated Box (KRAB) repressor domains are highly conserved polypeptide motifs and constitute about one third of all human zinc finger transcription factors. This suggests key regulatory features in higher eukaryotic transcriptomics. Even though KRAB minimal domains are usually no longer than ~ 50-75 amino acids, their mechanism of function remains complex. <br><br> Common biochemical models suggest a key role in epigenetic silencing, by recruiting a scaffold of diverse proteins - amongst others histone deacetylases and histone methyltransferases. Til date (2013), KRAB repressor domains were attached to several DNA binding proteins such as tetR, TAL effectors and [http://2013.igem.org/Team:Freiburg/Project/effector#repression dCas9] - thereby efficiently silencing gene expression downstream of desired target promoters.<br><br><br><br><br> | + | Krüppel-associated Box (KRAB) repressor domains are highly conserved polypeptide motifs and constitute about one third of all human zinc finger transcription factors. This suggests key regulatory features in higher eukaryotic transcriptomics (<i>Witzgall, R. et al.</i>, 1994). Even though KRAB minimal domains are usually no longer than ~ 50-75 amino acids, their mechanism of function remains complex. <br><br> Common biochemical models suggest a key role in epigenetic silencing, by recruiting a scaffold of diverse proteins - amongst others histone deacetylases and histone methyltransferases. Til date (2013), KRAB repressor domains were attached to several DNA binding proteins such as tetR, TAL effectors and [http://2013.igem.org/Team:Freiburg/Project/effector#repression dCas9] - thereby efficiently silencing gene expression downstream of desired target promoters.<br><br><br><br><br> |
<!-- Uncomment this to enable Functional Parameter display | <!-- Uncomment this to enable Functional Parameter display | ||
Line 44: | Line 44: | ||
<partinfo>BBa_K1150002 parameters</partinfo> | <partinfo>BBa_K1150002 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
+ | |||
+ | ==Literature== | ||
+ | <div id="(1)">(1) Rosati, M. <i>et al.</i> (1991). Members of the zinc finger protein gene family sharing a conserved N-terminal module. Nucleic acids research 19, <i>5661-5667</i>. <br></div> | ||
+ | <div id="(2)">(2) Witzgall, R. <i>et al.</i> (1994). The Krüppel-associated box-A domain of zinc finger proteins mediates transcriptional repression. Proc Nati Acad Sci 91, <i>4514-4518</i>. <br></div> | ||
+ | <div id="(3)">(3) Birtle, Z. and Ponting, C. (2006). Meisetz and the birth of the KRAB motif. Bioinformatics 22, <i>2841-2845</i>. <br></div> | ||
+ | <div id="(4)">(4) Urrutia, R. (2003). KRAB-containing zinc-finger repressor proteins. Genome Biology 4, <i>4:231</i>. <br></div> | ||
+ | <div id="(5)">(5) Fritz, R. and Radziwill, G. (2011). CNK1 and other scaffolds for Akt/FoxO signaling. Biochimica et biophysica acta 1813, <i>1971-1977</i>. <br></div> | ||
+ | <div id="(6)">(6) Bałan, B. and Słotwiński, R. (2008). VEGF and tumor angiogenesis. Centr Eur J Immunol 33, <i>232-236</i> <br></div></small> | ||
+ | |||
+ | </p> <br> |
Revision as of 16:20, 27 September 2013
KRAB
KRAB | |
---|---|
Function | Repressor domain |
Use in | Mammalian cells |
RFC standard | RFC 25 |
Backbone | pSB1C3 |
Organism | Homo sapiens |
Source | Konrad Müller, pKM102 |
Submitted by | [http://2013.igem.org/Team:Freiburg Freiburg 2013] |
Krüppel-associated Box (KRAB) repressor domains are highly conserved polypeptide motifs and constitute about one third of all human zinc finger transcription factors. This suggests key regulatory features in higher eukaryotic transcriptomics (Witzgall, R. et al., 1994). Even though KRAB minimal domains are usually no longer than ~ 50-75 amino acids, their mechanism of function remains complex.
Common biochemical models suggest a key role in epigenetic silencing, by recruiting a scaffold of diverse proteins - amongst others histone deacetylases and histone methyltransferases. Til date (2013), KRAB repressor domains were attached to several DNA binding proteins such as tetR, TAL effectors and [http://2013.igem.org/Team:Freiburg/Project/effector#repression dCas9] - thereby efficiently silencing gene expression downstream of desired target promoters.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 244
Illegal SapI.rc site found at 208
Literature
</p>