Difference between revisions of "Part:BBa J32009"

 
 
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<partinfo>BBa_J32009 short</partinfo>
 
<partinfo>BBa_J32009 short</partinfo>
  
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Intein splices itself out of a protein placed on N and C terminal ends in the presence of 4HT allowing for control protein activity in a dose dependent manner.
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Intein splices itself out of a protein placed on N and C terminal ends in the presence of 4HT allowing for control of protein activity in a dose dependent manner.  Shown to work in yeast.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:25, 6 September 2006

4HT Dependent Intein

Intein splices itself out of a protein placed on N and C terminal ends in the presence of 4HT allowing for control of protein activity in a dose dependent manner. Shown to work in yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 522
    Illegal PstI site found at 706
    Illegal PstI site found at 877
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1054
    Illegal PstI site found at 706
    Illegal PstI site found at 877
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 656
    Illegal XhoI site found at 1180
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 522
    Illegal PstI site found at 706
    Illegal PstI site found at 877
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 522
    Illegal PstI site found at 706
    Illegal PstI site found at 877
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 556