Difference between revisions of "Part:BBa K1202108:Experience"

 
Line 1: Line 1:
 +
_Inducible promoter experiment
 +
To observe inducible promoters response, we co incubated two different bacteria. The first bacteria culture is expressing enzyme system and the second culture is including inducible promoter. At the experiment, we incubated them seperately for 16 hours and mixed them into one flask and 4 hour later, we add 0,1 mM SAH (with 10x PBS containing %1 BSA). Incubate with SAH for one day and, took 2 ml of liquid culture to santrifuge tube.Centrifuged it. We saw the red color at the pellet as you see below.
 +
 +
So, it means the enzyme system is working and inducible promoter is succesfully induced from AI-2.
 +
Discussion
 +
  We were searching for inducible system that, getting activated with cancer cell antigen precense. As an activating molecule, autoinducer2 activates LsrR-LsrK promoter system for express the killer protein by bacteria.
 +
  Our co-incubation liquid cultures’ santrifuged tubes are seemin red colored.We added rfp gene to continue of inducible promotor gene and rfp is marker of activating. So this co incubation result significantly shows us promoter system is activating in the precense of autoindcer-2.
 +
  
−
__NOTOC__
 
−
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
−
how you used this part and how it worked out.
 
  
 
===Applications of BBa_K1202108===
 
===Applications of BBa_K1202108===

Revision as of 03:56, 5 October 2013

_Inducible promoter experiment To observe inducible promoters response, we co incubated two different bacteria. The first bacteria culture is expressing enzyme system and the second culture is including inducible promoter. At the experiment, we incubated them seperately for 16 hours and mixed them into one flask and 4 hour later, we add 0,1 mM SAH (with 10x PBS containing %1 BSA). Incubate with SAH for one day and, took 2 ml of liquid culture to santrifuge tube.Centrifuged it. We saw the red color at the pellet as you see below.

So, it means the enzyme system is working and inducible promoter is succesfully induced from AI-2. Discussion 

  We were searching for inducible system that, getting activated with cancer cell antigen precense. As an activating molecule, autoinducer2 activates LsrR-LsrK promoter system for express the killer protein by bacteria.
 Our co-incubation liquid cultures’ santrifuged tubes are seemin red colored.We added rfp gene to continue of inducible promotor gene and rfp is marker of activating. So this co incubation result significantly shows us promoter system is activating in the precense of autoindcer-2.


Applications of BBa_K1202108

User Reviews

UNIQcf5a993400eb4772-partinfo-00000000-QINU UNIQcf5a993400eb4772-partinfo-00000001-QINU