Difference between revisions of "Part:BBa K1140006:Design"

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Elowitz, MB and Leibler, S, (2000). A synthetic oscillatory network of transcriptional regulators, ''Nature'', 20;403(6767):335-8.
 
Elowitz, MB and Leibler, S, (2000). A synthetic oscillatory network of transcriptional regulators, ''Nature'', 20;403(6767):335-8.
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Neupert J, Karcher D, Bock R (2008) Design of simple synthetic RNA thermometers for temperature- controlled gene expression in Escherichia coli. "Nucleic Acids Res, 36(19):e124.

Revision as of 20:38, 24 September 2013

pTet + 37 oC RNA thermometer + mCherry (LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 63
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 676
    Illegal AgeI site found at 788
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.

Source

Obtained synthetically. The RFP variant employed is the same that is found in part BBa_E1010, which is a monomeric variant of the red fluorescent protein found in the coral Discosoma striata. The pTet promoter is identical to the sequence found in part BBa_R0040.

References

Elowitz, MB and Leibler, S, (2000). A synthetic oscillatory network of transcriptional regulators, Nature, 20;403(6767):335-8.

Neupert J, Karcher D, Bock R (2008) Design of simple synthetic RNA thermometers for temperature- controlled gene expression in Escherichia coli. "Nucleic Acids Res, 36(19):e124.