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| − | We constructed ATF2 generator. (BBa_K1049002)
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| − | Our team KIT-Kyoto 2013 constructed this part for the purpose of measurement. T7 promoter is an IPTG-inducible promoter. We added 20uL IPTG (100mM) to our genetically modified E.coli after cultivation at 28 and 37 degree C. 2 hours after, we extracted soluble proteins from it by using FastBreak™ Cell Lysis Reagent and did SDS-polyacrylamide gel electrophoresis.
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| − | [[File:KIT2013SDS.png|500px|thumb|left|Figure.1 SDS-PAGE Starting from the left, marker, ATF2 at 28 degree C, ATF2 + IPTG at 28 degree C, marker, ATF2 at 37 degree C, ATF2 + IPTG at 37 degree C]]
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| − | ATF2 gene encodes AATase, which is about 62kDa. The consumption of protein marker is like this.
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| − | Myosin 200kDa
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| − | β‐Galactosidase 120kDa
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| − | Bovine Serum Albumin 95kDa
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| − | Glutamine dehydrogenase 68kDa
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| − | Ovalbumin 50kDa
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| − | Carbonic Anhydrase 36kDa
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| − | Myoglobin 27kDa
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| − | Lysozyme 20kDa
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| − | Aprotinin 10kDa
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| − | You can find the band at lanes which are added IPTG just beneath the band of 68kDa.
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