Difference between revisions of "Part:BBa K1151019"
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<partinfo>BBa_K1151019 short</partinfo>
 
<partinfo>BBa_K1151019 short</partinfo>
  
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Combination of the following parts: BBa_K1151011 + BBa_K1151008. This combination displays red and green
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Combination of the following parts: [[BBa_K1151011]] + [[BBa_K1151008]]. This combination displays red and green fluorescence under specific conditions. GFP is costitutively expressed and it is possible to repress its expression usingnickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor. On the contrary RFP is switched off normally, but it can be switched on by the addiction of nickel ions in medium and in presence of HpNikR ([[BBa_K1151000]]) as trascription factor.
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fluorescence under specific conditions. GFP is costitutively expressed and it is possible to repress its expression using
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nickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor. On the contrary RFP is
+
−
switched off normally, but it can be switched on by the addiction of nickel ions in medium and in presence of HpNikR
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(BBa_K1151000) as trascription factor.
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and Features===
 
<partinfo>BBa_K1151019 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1151019 SequenceAndFeatures</partinfo>
  

Revision as of 11:03, 1 October 2013

GFP-RFP nickel regulated 3 (without terminator)

Combination of the following parts: BBa_K1151011 + BBa_K1151008. This combination displays red and green fluorescence under specific conditions. GFP is costitutively expressed and it is possible to repress its expression usingnickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor. On the contrary RFP is switched off normally, but it can be switched on by the addiction of nickel ions in medium and in presence of HpNikR (BBa_K1151000) as trascription factor.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 919