Difference between revisions of "Part:BBa K1073011"

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Note that the promoter is leaking to a certain degree when not activated. For further information and experience on this device see BBa_K1073035.
 
Note that the promoter is leaking to a certain degree when not activated. For further information and experience on this device see BBa_K1073035.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 18:10, 23 September 2013

Inducible ampicillin resistance cassette (regulated by RhlR + N-buturyl-HSL)

This device is a combination of BBa_K1073001 and BB_K1073000 and mediates a inducible ampicillin resistance. It includes the inducible promoter of the rhl quorum sensing system of Pseudomonas aeruginosa followed by a RBS and the ampicillin resistance gene coding for beta-lactamase.

Usage and Biology

The promoter is activated in the presence of RhlR in combination with the autoinducer molecule N-buturyl-homoserine lactone produced by RhlI. The expression of the beta-lactamase can therefore be induced and tuned by supplying RhlR and N-buturyl-HSL.

Note that the promoter is leaking to a certain degree when not activated. For further information and experience on this device see BBa_K1073035.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]