Difference between revisions of "Part:BBa K1045009"

Revision as of 08:39, 23 September 2013

Terminator BBa_B0015 with inversed Pre- and Suffix

This part is composed of an inverse terminator sequence BBa_B0015, i. e. the prefix sequence is located at the end of the terminator while the suffix sequence is placed in front of the terminator sequence. This inverse terminator could be used to stop transcription of inverse coding sequences, such as BBa_K1045001 (inverse DarR). In particular, this terminator might be helpful if you want to clone two genes into one vector so that both genes are transcribed into diverging directions.
BBa_K10450009 was generated by amplifying part BBa_B0015 from a plasmid using a forward primer harboring the suffix sequence and a reverse primer fused to the prefix sequence.

Secondary Structure

File:Mfold-K1045009-1.png

Measurement

[http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

Terminator BBa_B0015 with inversed Pre- and Suffix

Secondary Structure

File:Mfold-K1045009-1.png

Measurement

[http://openwetware.org/wiki/Cconboy:Terminator_Characterization/Results How these parts were measured]

@@ Line 8: / Line 8: @@
 <partinfo>BBa_K1045009 short</partinfo>
 * This part is composed of an inverse terminator sequence BBa_B0015, i. e. the prefix sequence is located at the end of the terminator while the suffix sequence is placed in front of the terminator sequence. This inverse terminator could be used to stop transcription of inverse coding sequences, such as BBa_K1045001 (inverse DarR). In particular, this terminator might be helpful if you want to clone two genes into one vector so that both genes are transcribed into diverging directions.
+* BBa_K10450009 was generated by amplifying part BBa_B0015 from a plasmid using a forward primer harboring the suffix sequence and a reverse primer fused to the prefix sequence.
 |}