Difference between revisions of "Part:BBa R0011"

Revision as of 19:10, 17 July 2006

Promoter (lacI regulated, lambda pL hybrid)

Inverting regulatory region controlled by LacI (BBa_C0010, BBa_C0012, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be:

repressed by LacI, the Lac inhibitor (i.e. repressor) (BBa_C0012) ([LUTZ97]).
induced by [http://openwetware.org/wiki/IPTG IPTG] in E.Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range

Usage and Biology

Strong promoter. [jb, 5/24/04]

R0011 will be on in strains without lacI, off in strains that are lacIq and medium in strains that are lacI

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_R0011 short</partinfo>
 Inverting regulatory region controlled by LacI (<bbpart>BBa_C0010</bbpart>, <bbpart>BBa_C0012</bbpart>, etc.)
+The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1.
-The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bbpart>BBa_C0012</bbpart>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by [http://openwetware.org/wiki/IPTG IPTG] in E.Coli DH5-alpha-Z1 (same paper reference).
+The hybrid design allows for strong promotion that can nevertheless be:
+*repressed by LacI, the Lac inhibitor (i.e. repressor) (<bbpart>BBa_C0012</bbpart>) ([LUTZ97]).
+*induced by [http://openwetware.org/wiki/IPTG IPTG] in E.Coli DH5-alpha-Z1 (same paper reference) over a >600-fold range
@@ Line 11: / Line 12: @@
 Strong promoter. [jb, 5/24/04]
+R0011 will be on in strains without lacI, off in strains that are lacIq and medium in strains that are lacI
 <span class='h3bb'>Sequence and Features</span>