Difference between revisions of "Part:BBa K1080000:Design"
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===Design Notes=== | ===Design Notes=== | ||
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+ | Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence | ||
===Source=== | ===Source=== |
Revision as of 01:40, 23 September 2013
ChlI1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 231
Illegal BglII site found at 887
Illegal BglII site found at 1082
Illegal BamHI site found at 279 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Incorporated sequence overlap for Gibson assembly and no GC rich region or restriction site in sequence
Source
Chlamydomonas reinhardtii