Difference between revisions of "Part:BBa K1163111"
 
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<partinfo>BBa_K1163111 short</partinfo>
 
<partinfo>BBa_K1163111 short</partinfo>
  
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Promoter from E. coli that controls the yncE gene, involved in iron uptake.  
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Promoter from E. coli that controls the YncE gene, involved in iron uptake.  
  
 
This promoter sequence contains a RBS and a FUR binding site, although it is not clearly possible to identify them precisely. It has been shown to downregulate the expression of sfGFP or any gene down-stream in the presence of iron in the range: 10<sup>-7</sup> to 10<sup>-4</sup> mol.L-1  
 
This promoter sequence contains a RBS and a FUR binding site, although it is not clearly possible to identify them precisely. It has been shown to downregulate the expression of sfGFP or any gene down-stream in the presence of iron in the range: 10<sup>-7</sup> to 10<sup>-4</sup> mol.L-1  
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Why would you use this part?  
 
Why would you use this part?  
  
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This part allows the characterization of the yncE promoter region thanks to the sfGFP downstream.
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This part allows the characterization of the YncE promoter region thanks to the sfGFP downstream.
  
  

Latest revision as of 21:05, 22 September 2013

YncE-sfGFP-Term

Promoter from E. coli that controls the YncE gene, involved in iron uptake.

This promoter sequence contains a RBS and a FUR binding site, although it is not clearly possible to identify them precisely. It has been shown to downregulate the expression of sfGFP or any gene down-stream in the presence of iron in the range: 10-7 to 10-4 mol.L-1

FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron FUR will be linked to the iron. This modification of conformation will induce a dimerization of FUR. Then the dimeric FUR will bind to the DNA in a Fur Binding Site and inhibit the mRNA transcription.

To caracterize our constructions we use this Fur binding site merged with a super fold GFP to the power of inhibition of the Fur binding site.

Why would you use this part?

This part allows the characterization of the YncE promoter region thanks to the sfGFP downstream.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 319