Difference between revisions of "Part:BBa K1163102"

Revision as of 20:47, 22 September 2013

pAceB-sfGFP-Term

Promoter from E. coli that controls the AceB gene, involved in iron uptake.

This promoter sequence contains a RBS and a FUR binding site, although it is not clearly possible to identify them precisely. It has been shown to downregulate the expression of sfGFP or any gene down-stream in the presence of iron in the range: 10^-7 to 10^-4 mol.L^-1

FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron FUR will be linked to the iron. This modification of conformation will induce a dimerization of FUR. Then the dimeric FUR will bind to the DNA in a Fur Binding Site and inhibit the mRNA transcription.

To realize our project we want to use this Fur binding site merged with an inhibitor (here LacI) to create an inverter system.

AceB regulatory promoter will decrease the expression of the LacI-LVA gene (LacI protein with a LVA degredation tag) when the FUR protein is dimerized in the presence of iron. This part should be used to express a gene of interest that is under control of a Lac operator (LacO). This way, the gene will be positively regulated in the presence of iron.

Why would you use this part?

This part allows the characterization of the AceB promoter region thanks to the sfGFP downstream.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 277
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 319

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1163102 short</partinfo>
-Promoter from E. coli that controls the AceB gene, involved in iron uptake and iron sensitive.
+Promoter from E. coli that controls the AceB gene, involved in iron uptake.
+This promoter sequence contains a RBS and a FUR binding site, although it is not clearly possible to identify them precisely. It has been shown to downregulate the expression of sfGFP or any gene down-stream in the presence of iron in the range: 10<sup>-7</sup> to 10<sup>-4</sup> mol.L<sup>-1</sup>
+FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron FUR will be linked to the iron. This modification of conformation will induce a dimerization of FUR. Then the dimeric FUR will bind to the DNA in a Fur Binding Site and inhibit the mRNA transcription.
+To realize our project we want to use this Fur binding site merged with an inhibitor (here LacI) to create an inverter system.
+AceB regulatory promoter will decrease the expression of the LacI-LVA gene (LacI protein with a LVA degredation tag) when the FUR protein is dimerized in the presence of iron. This part should be used to express a gene of interest that is under control of a Lac operator (LacO). This way, the gene will be positively regulated in the presence of iron.
-FUR (Ferric Uptake Regulation) is a transcriptional repressor of genes involved in iron homeostasis. In presence of iron, the FUR protein changes its conformation and dimerizes. This leads to the binding to the DNA on a FUR binding site, thus inhibiting the mRNA transcription.
 Why would you use this part?