Difference between revisions of "Part:BBa K1065310"

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<partinfo>BBa_K1065310 short</partinfo>
 
<partinfo>BBa_K1065310 short</partinfo>
  
This part consists of a complete Blue receptor protein and a Cl inverter, which are needed to produce chromoprotein (amilCP) under a blue light (470nm in our case).
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This part is a blue light sensible device: it consists of a Blue light sensor with its response regulator (Bba_K592016), the RR dependent promoter (Bba_K592006) and an inverter cassette (cI and pLambda) which are needed to produce the reporter (amilCP) when blue light (470 nm) is present. Dark causes the turning off, thus inhibits the production of the reporter. Everything is under the control of a constitutive promoter (anderson family).
 
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This part was cloned and successfully characterized by UNITN-Trento 2013 iGEM team in order to test protein transcription and then replace the blue chromoprotein with an ethylene forming enzyme (EFE). Parts from 2011 Uppsala-Sweden team and 2006 Berkeley team were used.
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 06:13, 23 September 2013

Blue light circuit with inverter for the production of amilCP

This part is a blue light sensible device: it consists of a Blue light sensor with its response regulator (Bba_K592016), the RR dependent promoter (Bba_K592006) and an inverter cassette (cI and pLambda) which are needed to produce the reporter (amilCP) when blue light (470 nm) is present. Dark causes the turning off, thus inhibits the production of the reporter. Everything is under the control of a constitutive promoter (anderson family). This part was cloned and successfully characterized by UNITN-Trento 2013 iGEM team in order to test protein transcription and then replace the blue chromoprotein with an ethylene forming enzyme (EFE). Parts from 2011 Uppsala-Sweden team and 2006 Berkeley team were used.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 605
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 767
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1297
    Illegal NgoMIV site found at 1590
    Illegal NgoMIV site found at 1684
    Illegal AgeI site found at 319
    Illegal AgeI site found at 1465
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1354
    Illegal BsaI.rc site found at 218