Difference between revisions of "Part:BBa K1150024:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
 
For usage in mammalian systems this device contains two strong, viral NLS, that bring the protein into the nucleus. An HA-tag was cloned N-terminal to detect it by Western Blot or ELISA approaches. A short linker seperates the dCAS9 and the G9a-SD to minimize the possibility of interference in between the domains.
 
For usage in mammalian systems this device contains two strong, viral NLS, that bring the protein into the nucleus. An HA-tag was cloned N-terminal to detect it by Western Blot or ELISA approaches. A short linker seperates the dCAS9 and the G9a-SD to minimize the possibility of interference in between the domains.
 +
[[File:Freiburg2013_Plasmid_Cas9-G9a.png|800px|]] <br>
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'''Figure 1.:''' Complete overview on CMV:dCas9-G9a with all features.
  
 
===Source===
 
===Source===

Revision as of 23:07, 1 October 2013


uniCAS Histone Modifier (CMV promoter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 576
    Illegal BglII site found at 900
    Illegal BglII site found at 5375
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For usage in mammalian systems this device contains two strong, viral NLS, that bring the protein into the nucleus. An HA-tag was cloned N-terminal to detect it by Western Blot or ELISA approaches. A short linker seperates the dCAS9 and the G9a-SD to minimize the possibility of interference in between the domains. Freiburg2013 Plasmid Cas9-G9a.png
Figure 1.: Complete overview on CMV:dCas9-G9a with all features.

Source

..

References