Difference between revisions of "Part:BBa K1151000:Design"

 
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===References===
 
===References===
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1.  Muller et al, Hierarchical regulation of the NikR-mediated nickel response in Helicobacter pylori. Nucleic Acids Res. 2011; 39(17):7564-75.
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2.  Dian  et al,  Structural Basis of the Nickel Response in Helicobacter pylori: Crystal Structures of HpNikR in Apo and Nickel -bound States.  J Mol Biol. 2006;
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25;361(4):715-30.
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3.  Dosanjh et Michel, Microbial nickel metalloregulation: NikRs for nickel ions. Curr Opin Chem Biol. 2006 (2):123-30.
 +
4.  Ernst  et al,  The nickel-responsive regulator NikR controls activation and repression of gene transcription in Helicobacter pylori .  Infect Immun.  2005;
 +
73(11):7252-8.

Revision as of 07:38, 26 September 2013


Nickel-responsive pleiotropic regulator (HpNikR)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It was inserted in a pET-15b plasmid vector, in order to have a better storage.


Source

It was amplified by PCR from the genomic sequence of Helicobacter pylori G27.

References

1. Muller et al, Hierarchical regulation of the NikR-mediated nickel response in Helicobacter pylori. Nucleic Acids Res. 2011; 39(17):7564-75. 2. Dian et al, Structural Basis of the Nickel Response in Helicobacter pylori: Crystal Structures of HpNikR in Apo and Nickel -bound States. J Mol Biol. 2006; 25;361(4):715-30. 3. Dosanjh et Michel, Microbial nickel metalloregulation: NikRs for nickel ions. Curr Opin Chem Biol. 2006 (2):123-30. 4. Ernst et al, The nickel-responsive regulator NikR controls activation and repression of gene transcription in Helicobacter pylori . Infect Immun. 2005; 73(11):7252-8.