Difference between revisions of "Part:BBa K1017301"

Revision as of 02:29, 21 September 2013

Cph8(cph1-envZ)

Cph8 is a chimeric light receptor. It is a fusion of the photoreceptor Cph1(pink) and the EnvZ histidine kinase. The light sensing unit is connected to transcription via the EnvZ-OmpR signaling pathway, phosphorylated OmpR acting as a transcription factor of PompC. Cph1 is only active when it binds the chromophore phycocyanobiline (PCB). Far red light(or darkness) activates the EnvZ kinase which results in the phosphorylation of OmpR and its activation as a transcription factor. On the other hand, red light deactivates the kinase and therefore the genes downstream can not be expressed. Take the image above as an example, the phosphorylated OmpR will turn on PompC then lacZ gene is translated, signaling out black pigment in the dark.

Sequence and Features

Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 364
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1017301 short</partinfo>
-https://static.igem.org/mediawiki/igem.org/2/21/NCTU_Formosa_part_3.png
+[[File:NCTU Formosa part 3.png|400px|center|400px]]
-Cph8 is a chimeric light receptor.  It is a fusion of the photoreceptor Cph1(pink) and the EnvZ histidine kinase.  The light sensing unit is connected to transcription via the EnvZ-OmpR signaling pathway, phosphorylated OmpR acting as a transcription factor of PompC. Cph1 is only active when it binds the chromophore phycocyanobiline (PCB). Far red light(or darkness) activates the EnvZ kinase which results in the phosphorylation of OmpR and its activation as a transcription factor. On the other hand, red light deactivates the kinase and therefore the genes downstream can not be expressed.
+Cph8 is a chimeric light receptor.  It is a fusion of the photoreceptor Cph1(pink) and the EnvZ histidine kinase.  The light sensing unit is connected to transcription via the EnvZ-OmpR signaling pathway, phosphorylated OmpR acting as a transcription factor of PompC. Cph1 is only active when it binds the chromophore phycocyanobiline (PCB). Far red light(or darkness) activates the EnvZ kinase which results in the phosphorylation of OmpR and its activation as a transcription factor. On the other hand, red light deactivates the kinase and therefore the genes downstream can not be expressed. Take the image above as an example, the phosphorylated OmpR will turn on PompC then lacZ gene is translated, signaling out black pigment in the dark.