Difference between revisions of "Part:BBa K1022121:Design"
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<partinfo>BBa_K1022121 short</partinfo> | <partinfo>BBa_K1022121 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
The Biobrick Bba_K1022105 is PCRed using designed primer to remove the pT7 promoter and then Ligated with the Biobrick Bba_K1022119 | The Biobrick Bba_K1022105 is PCRed using designed primer to remove the pT7 promoter and then Ligated with the Biobrick Bba_K1022119 | ||
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+ | '''Please visit [http://2013.igem.org/Team:TU-Delft/Planning iGEM 2013 Team TU Delft] ''' | ||
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Latest revision as of 15:47, 4 October 2013
pBAD: Agr A : Agr C : pP2 : RBS: His6- SUMO: Signiferin: RBS TetR :TT
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 2317 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 521
Illegal BglII site found at 2441
Illegal BamHI site found at 65
Illegal BamHI site found at 1405 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2626
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The Biobrick Bba_K1022105 is PCRed using designed primer to remove the pT7 promoter and then Ligated with the Biobrick Bba_K1022119
Please visit [http://2013.igem.org/Team:TU-Delft/Planning iGEM 2013 Team TU Delft]
Source
Clonned