Difference between revisions of "Part:BBa K1100006:Design"
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===Source=== | ===Source=== | ||
| − | + | Insulator: Csy4 loci via PCR, | |
| − | + | B0034 via PCR, | |
| + | sfGFP from Ding Yu's Lab at Fudan University | ||
===References=== | ===References=== | ||
Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. | Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006. | ||
Latest revision as of 10:19, 20 September 2013
optimized sfGFP with Csy4 insulator loci
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A optimized sfGFP with a Csy4 loci, can be used as a powerful reporter. You can get this part from BBa_K1100015 and other parts we have submitted.
Source
Insulator: Csy4 loci via PCR, B0034 via PCR, sfGFP from Ding Yu's Lab at Fudan University
References
Qi L, Haurwitz R E, Shao W, et al. RNA processing enables predictable programming of gene expression[J]. Nature biotechnology, 2012, 30(10): 1002-1006.