Difference between revisions of "Part:BBa K1045014:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1045014 short</partinfo>
 
<partinfo>BBa_K1045014 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
To be continued
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This part was constructed using hybridization oligos for BBa_K1045011. The hybridization product corresponded to a DNA fragment harboring the sequence of BBa_K10450011 cut with EcoRI and SpeI at the prefix and suffix sites. This fragment was ligated into BBa_K1045013 in a prefixing composition.
  
  

Revision as of 13:44, 23 September 2013

Promoter reverse - Promoter - DarR operator - GFP generator BBa_E0240


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
    Illegal NheI site found at 24
    Illegal NheI site found at 104
    Illegal NheI site found at 127
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 55
    Illegal AgeI site found at 963
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 825


Design Notes

This part was constructed using hybridization oligos for BBa_K1045011. The hybridization product corresponded to a DNA fragment harboring the sequence of BBa_K10450011 cut with EcoRI and SpeI at the prefix and suffix sites. This fragment was ligated into BBa_K1045013 in a prefixing composition.


Source

To be continued

References