Difference between revisions of "Part:BBa K1049002:Experience"
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===Applications of BBa_K1049002=== | ===Applications of BBa_K1049002=== | ||
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| + | Our team KIT-Kyoto 2013 constructed this part for the purpose of measurement. | ||
| + | T7 promoter is an IPTG-inducible promoter. | ||
| + | We added 20uL IPTG (100mM) to our genetically modified E.coli after cultivation at 28 and 37 degree C. | ||
| + | 2 hours after, we extracted soluble proteins from it by using FastBreak™ Cell Lysis Reagent and did SDS-polyacrylamide gel electrophoresis. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 02:08, 20 September 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1049002
Our team KIT-Kyoto 2013 constructed this part for the purpose of measurement. T7 promoter is an IPTG-inducible promoter. We added 20uL IPTG (100mM) to our genetically modified E.coli after cultivation at 28 and 37 degree C. 2 hours after, we extracted soluble proteins from it by using FastBreak™ Cell Lysis Reagent and did SDS-polyacrylamide gel electrophoresis.
User Reviews
UNIQ1bcf22179df6d5cb-partinfo-00000000-QINU UNIQ1bcf22179df6d5cb-partinfo-00000001-QINU