Difference between revisions of "Part:BBa K1111012:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | + | ==Applications of BBa_K1111012== | |
| + | This part can be used in any experience with biotin. Biotinylation of biological material is widely used and quick to do. Once biotinylated, the molecule will be able to attach to the streptavidin expressed in bacteria. For us, the material was nanoparticles, that we nicely biotinylated [http://2013.igem.org/Team:EPF_Lausanne/Nanoparticles] | ||
| + | ==Sequencing== | ||
| + | We sequenced this part once the Gibson assembly made. To do so, we used primers for iGEM sites VF2 and VR, in order to sequence all that was inserted in the backbone pSB1C3. | ||
| + | The sequencing results are shown here : | ||
| + | |||
| + | ==Microscopy== | ||
| + | Also, we transformed cells and used them to do microscopy. Two experiments were done, one with a fluorescent antibody against streptavidin and the other one with a fluorescent biotin. We did both in case the antibody would be too big, since biotin is a small molecule best suit to cross glycocalix. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 11:03, 2 October 2013
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1111012
This part can be used in any experience with biotin. Biotinylation of biological material is widely used and quick to do. Once biotinylated, the molecule will be able to attach to the streptavidin expressed in bacteria. For us, the material was nanoparticles, that we nicely biotinylated [http://2013.igem.org/Team:EPF_Lausanne/Nanoparticles]
Sequencing
We sequenced this part once the Gibson assembly made. To do so, we used primers for iGEM sites VF2 and VR, in order to sequence all that was inserted in the backbone pSB1C3. The sequencing results are shown here :
Microscopy
Also, we transformed cells and used them to do microscopy. Two experiments were done, one with a fluorescent antibody against streptavidin and the other one with a fluorescent biotin. We did both in case the antibody would be too big, since biotin is a small molecule best suit to cross glycocalix.
User Reviews
UNIQbaa1f2d03589c0d3-partinfo-00000000-QINU UNIQbaa1f2d03589c0d3-partinfo-00000001-QINU