Difference between revisions of "Part:BBa K1111007:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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how you used this part and how it worked out.
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EPF_Lausanne characterization of the part
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After the plasmid was complete, bacteria were transformed with it and plated. A colony was then picked and the plasmid was sent for sequencing while a glycerol stock of the colony was made. After the sequence was verified, part of the stock was inoculated overnight and then induced by adding 50ul of 20% arabinose to the 5ml liquid culture medium, the expected result being secretion (or at least expression) of the MMP2 protein. A Western blot was performed with anti-His tag antibodies on the super stand and lysate of the bacteria. The result was unfortunately negative. A His-tag purification was then performed and followed by an SDS-PAGE.
  
 
===Applications of BBa_K1111007===
 
===Applications of BBa_K1111007===
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===User Reviews===
 
===User Reviews===

Revision as of 21:30, 30 September 2013


EPF_Lausanne characterization of the part After the plasmid was complete, bacteria were transformed with it and plated. A colony was then picked and the plasmid was sent for sequencing while a glycerol stock of the colony was made. After the sequence was verified, part of the stock was inoculated overnight and then induced by adding 50ul of 20% arabinose to the 5ml liquid culture medium, the expected result being secretion (or at least expression) of the MMP2 protein. A Western blot was performed with anti-His tag antibodies on the super stand and lysate of the bacteria. The result was unfortunately negative. A His-tag purification was then performed and followed by an SDS-PAGE.

Applications of BBa_K1111007

User Reviews

UNIQa5c27f13d48836a5-partinfo-00000000-QINU UNIQa5c27f13d48836a5-partinfo-00000001-QINU