Difference between revisions of "Part:BBa K1140006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI. | + | The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI. |
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===Source=== | ===Source=== | ||
Revision as of 04:53, 24 September 2013
pTet + 37 oC RNA thermometer + mCherry (LVA)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 63
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 676
Illegal AgeI site found at 788 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The prefix sites are EcoRI and XbaI and the sufix sites are SpeI and PstI.
Source
Obtained synthetically. The RFP variant employed is the same that is found in part BBa_E1010, which is a monomeric variant of the red fluorescent protein found in the coral Discosoma striata. The pTet promoter is identical to the sequence found in part BBa_R0040.