Difference between revisions of "Part:BBa K1140006"

Revision as of 20:55, 25 September 2013

pTet + 37 oC RNA thermometer + mCherry (LVA)

mCherry coding sequence is under the regulation of a pTet promoter. Translation is also regulated by a synthetic RNA thermometer[1] that allows translation only over 37 °C.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 63
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 676
Illegal AgeI site found at 788
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1140006 short</partinfo>
-Codifies for a RFP with a LVA tag. The RFP is under the regulation of a pTet promoter and a thermolabile ribosome binding site with an optimal translation temperature of 37 °C. Two transcription termination sites were also added at the 3’ end of the gene.
+mCherry coding sequence is under the regulation of a pTet promoter. Translation is also regulated by a synthetic RNA thermometer[1] that allows translation only over 37 °C.
 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+RNA thermometers (RNAT) are temperature sensing RNA sequences that regulate translation by preventing the ribosome from binding the transcript until higher temperatures shift it to an open structure. Several naturally occurring RNA thermometers have been described and synthetic sequences that emulate them have been designed and proved to regulate genetic expression at different temperature ranges. The RNAT herein implemented, designed and dubbed u6 by Neupert ''et al.'' [1], is a synthetic sequence that allows translation only at temperatures over 37 °C. This part can be incorporated into a genetic circuit to integrate transcriptional  and post-transcriptional regulation, through the Tet Repressor and u6-RNAT, respectively.
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