Difference between revisions of "Part:BBa K1092104"
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<partinfo>BBa_K1092104 short</partinfo> | <partinfo>BBa_K1092104 short</partinfo> | ||
− | + | An RFP fragment adapted from the RFP part BBa_E1010, and its counterpart is the RFP N terminus (BBa_K1092105). It is adapted to be expressed downstream of a fusion protein such as our Voltage switch parts (BBa_K1092023 & BBa_K1092024). It is the C terminal of the RFP, and is capable of refolding with it’s counter part N terminal as demonstrated by the NYMU-Taipei 2010 iGEM team through the Biomolecular Fluorescent complementation (BiFC) to give fluorescent (BBa_K411103 & BBa_K411104). We adapt this protein to be the downstream of our voltage switch because we want it to become our effector of the switch. | |
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Revision as of 22:15, 27 September 2013
T7-QsrR binding site-RBS
An RFP fragment adapted from the RFP part BBa_E1010, and its counterpart is the RFP N terminus (BBa_K1092105). It is adapted to be expressed downstream of a fusion protein such as our Voltage switch parts (BBa_K1092023 & BBa_K1092024). It is the C terminal of the RFP, and is capable of refolding with it’s counter part N terminal as demonstrated by the NYMU-Taipei 2010 iGEM team through the Biomolecular Fluorescent complementation (BiFC) to give fluorescent (BBa_K411103 & BBa_K411104). We adapt this protein to be the downstream of our voltage switch because we want it to become our effector of the switch.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]