Difference between revisions of "Part:BBa K1055001"
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+ | '''Characterization LssmOrange''' | ||
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+ | This biobrick did measure up to our expectations as shown in the following data. We also used this promoter to express our PET cleaving enzyme pNB-Est13, which is anchored C-terminal to EstA.(''E. Coli'' membrane anchor protein). AraC-Pbad shows a low background activity and a good respose to the induction with Arabinose. | ||
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+ | [[Image:Spectra_LssmOrange.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]] | ||
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+ | [[Image:MKate-LSSmOrange_FRET_Pair.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]] | ||
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+ | [[Image:LssmOrange_bac.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]] | ||
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+ | [[Image:Grow_log.png|400px|thumb|Figure 1. '''Cells grown in GFP-medium containing the arabinose promoter regulating GFP expression.''' Induced at time=0h.]] |
Revision as of 21:37, 4 October 2013
LssmOrange is a fluorophor derived from dsRed
LssmOrange is a fluorophor derived from dsRed. It features the excitation maxima at 437 nm and the emission maxima at 571 nm.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 570
- 1000COMPATIBLE WITH RFC[1000]
Characterization LssmOrange
This biobrick did measure up to our expectations as shown in the following data. We also used this promoter to express our PET cleaving enzyme pNB-Est13, which is anchored C-terminal to EstA.(E. Coli membrane anchor protein). AraC-Pbad shows a low background activity and a good respose to the induction with Arabinose.